Presenters:
Lecturers and those who are joining today i am nurse sabrina and along with mr faziana we are going to present our research with title decide to toss the effects of novel name to its foreign nasopharyngeal carcinoma and in vitro study which are supervised by the as doctor muhammad and sir muhammad abdul rahim human esophageal carcinoma also known as npc is one
Of the most common head and necklaces with the highest incidence and mortality rates in asian countries including malaysia based on world health organization who in 2020 nasopharynx cancer is listed at top 5 for new cases and top 6 for the death in malaysia despite the fact that majority of patients have been cured there are still challenges in prevention of
The disease relapse treatments of patients with refractory or metastatic mpc and also management of long-term of toxicities so in our study we use investigating to treat this cancer symbol setting is cholesterol lowering drug which belongs to the statin family and it is widely used as a safe and well tolerated therapeutic agents statins have been shown to have
Anti-proliferative and apoptotic activity against variety of cancer cell lines including breast prostate and colon cancers simvastatin has gained a lot of attention because it has the potential to be anti-carcinogenic when compared to the other state however civil setting alone may cause cancer cells to develop chemo resistance to prevent this from happen we
Decided to encapsulate the simvastatin into micro particles with size ranging plus minus 1 200 micrometer the reason why we choose to encapsulate it because the micro particles will help to protect the drug which is the symbol setting from environment protect the body from the side effects and also it will improve the effect of the drug in addition kaitosan also
Is added into this study as coating material to the microparticles kaitosan helps to modify the drug release behavior of microparticles with moderate and sustain release okay so next is our objective we would like to investigate the effects of encapsulated symmetry loaded microparticles as antiproliferative agents on human esophageal carcinoma by using rpm i2650
Cell line moving on to our methodology the simvastatin microparticles are first prepared using a double imaging solvent evaporation technique and then these samples were observed under scanning electron microscope imaging sem and high performance liquid chromatography hplc sem helps to observe the morphology of the microparticles so these are the expectation
Results where there are non-porous microparticles with varying sizes next is hplc we use this to evaluate the encapsulation efficiency and also to evaluate the drug loading simvastatin nanporus microparticles then i2 650 cell line were cultured and treated with these four types of samples with various concentrations which are simvastatin alone t1 plg is similar
Setting microparticles t2 the lda symbol stating chitosan microparticles t3 and lastly the positive control system t4 these samples then were assessed with mttac with different time mana which are 24 hours 48 hours and 72 hours these data then were converted into percentage of sub-ability and were analyzed with repeated measure of variance to identify their
Significant differences within groups assalamu alaikum and good morning i am norfazliana i will continue with the result part the first part is microparticle preparation this image showing are the result of nonporous microparticle that is varied in size distribution observed underscoring electron microscope as we can see in the first image is the different sizes
Of simvastatin microparticles in one milligram per mil the second image is invested in micro particles in two milligram per ml and the third image is invested in micro particle in three milligram per mil while the last image showing is invested in micro particles that is coated with kaitosan moving on to the cell viability result using mttsa the time observation
Of this study were made at 24 hours 48 hours and 72 hours studies showed that cell viability decreased in rpm i-2650 cells line that is treated with t1 t2 t3 in concentration time-dependent manner as we can see in the graph showing the cells that is treated with synvested in a wound which is t1 should a great cell reduction after 72 hours of treatment with one
Two and three milligram per meal from one hundred percent to zero point one seven percent zero point four three percent and zero point four four percent respectively in t1 the reduction in one two and three milligram per mil is significant as compared in 24 48 and 72 hours x for the cell treated with plgas invested in microparticle which is t2 we can see in the
Graph that t2 showed a decrease in cell viability in 24 48 and 72 hours cell growth reduction was seen greatly after 72 hours of treatment with one two and three milligram per ml of t2 in which the cell viability decreased from hundred percent to 29.84 percent 24.19 and 30.27 respectively in t2 the reduction in one two and three milligram per mil is significant
As compared in 24 48 and 72 hours moving on to the cells treated with plgas invested in kyto sun micro particles which is t3 we can see in the graph that the group of cells treated showed decreased cell growth reduction in slow manners as compared to t1 and t2 just now it showed cell growth reduction after 72 hours of treatment with one two and three milligram
Per meal of t3 from one hundred percent to seventy six point three four percent sixty eight point six five percent and sixty five point nine two percent respectively in t3 the reduction in one two and three milligram per meal is significant as compared in 24 48 and 72 hours this graph showed sample with various concentration at different time points as we can
See simvastatin showed the highest acceleration of killing effects followed by simvastatine microparticles and simvastatin microparticles that is coated with cytosine the study found that there is significant difference between three different samples in 24 48 and 72 hours using repeated measured anova next for statistical analysis repeated measure analysis of
Variance was applied for analysis of anti-cancer properties at different time points there was a significant difference of mean cell viability within each sample group on time it also showed that there was a significant difference in comparison between cells treated with t1 t2 t3 and control group against three different time points for analysis of anti-cancer
Properties within treated group regardless of time effect the test between subject effects showed that there was significant difference of mean cell viability among 4 samples with p-value less than 0.001 regardless of time for analysis of anti-cancer properties between treated group in 24 48 and 72 hours it shows that there was significant difference of mean cell
Viability among three different samples t1 t2 and t3 based on time and concentration with p-value less than 0.001 moving on to the discussion part there are extensive literature on the pleotropic effect of symbiostatin against nesopharyngeal carcinoma however by encapsulating simvastatin in the micro particles could be another option in anti-cancer delivery to
The targeting site in this study it is conclusively showed both t2 and t3 showed cytotoxic effect against nasopharyngeal carcinoma cell growth in a slow release manner as compared to the treatment with simvastatin alone moreover coating the symbiostatine plga microparticles with chitosan significantly sustained the symbiostatine release this is desirable action
Of drugs to reduce the side effects of simvastatin hence t2 and t3 showed a good option in delivering anti-cancer drug with a great association in reduction of tumor cell growth and cell death we would like to highlight the novelty of this work study of symbiostatine or nasopharyngeal carcinoma had received gold award in iidex 2019 and bronze award in irdex 2020.
This is pattern from the itm for micro x boon tissue grafting material as the preparation for microparticle is similar in conclusion this study demonstrated that simvastatin microparticles is associated with reduction of tumor cell growth and death in addition fabrication of microparticle with kaitosan will not only reduce the number of cell viability but also
Reduce the release of simvastatin which is useful in drug delivery strategy for anti-cancer treatment study conclusively shown that there is potentially therapeutic intervention of anti-cancer using simvastatin micro particles with kaitosan as coating material this is our references that is all from us thank you
Transcribed from video
RL-1-09:The Cytotoxic Effect Of NP Simvastatin Microparticles Coated With Chitosan Against Human NPC By UiTM Dental Students’ Scientific Symposium 2021
